The rapid development of digital technologies and the expansion of global online communication have significantly increased the demand for website localization across different linguistic and cultural environments. Localization extends beyond the traditional concept of translation by adapting digital content to the linguistic norms, cultural values, communicative expectations, and technical requirements of the target audience. This article examines the linguistic and cultural adaptation challenges encountered in the localization of English-language website content into Uzbek. Particular attention is given to lexical, grammatical, semantic, pragmatic, and sociocultural issues that emerge during the localization process. The study also explores the influence of cultural references, user experience considerations, and technological factors on localization quality. Through a comprehensive analysis of theoretical perspectives and practical examples, the research highlights the necessity of combining linguistic competence with cultural awareness to achieve effective localization outcomes. The findings demonstrate that successful localization contributes not only to accurate information transfer but also to enhanced user engagement, intercultural communication, and digital accessibility.