The study aimed to explore the effectiveness of employing AI Praat acoustic analysis to investigate the challenges Omani EFL college students face in producing English prosodic features, particularly sentence intonation and lexical stress. The test was developed and applied to thirty students from foundation programs at a university level. Students' audio-recordings were analyzed using Praat acoustic analysis. Findings indicated that Praat acoustic analysis are very effective as it provided precise and objective methods for examining prosodic features of sentence intonation and lexical stress. The detailed acoustic data generated by Praat helped in diagnosing phonetic acoustic issues and enabled the comparison of learners' speech with native speakers' norms, fostering a deeper understanding of the challenges faced by Omani EFL learners in mastering English prosody. The results revealed significant differences between the students' intonation patterns and lexical stress placement compared to native English speakers, likely influenced by mother tongue.