The Trail Making Test (TMT) is used in neuropsychological clinical practice to assess aspects of attention and executive function. The test consists of two parts (A and B) and requires drawing a trail between elements. Many patients are assessed with their non-dominant hand because of motor dysfunction that prevents them from using their dominant hand. Since drawing with the non-dominant hand is not an automatic task for many people, we explored the effect of hand use on TMT performance. The TMT was administered digitally in order to analyze new outcome measures in addition to total completion time. In a sample of 82 healthy participants, we found that non-dominant hand use increased completion times on the TMT B but not on the TMT A. The average completion time increased by almost 5 seconds, which may be clinically relevant. A substantial number of participants who performed the TMT with their non-dominant hand had a B/A ratio score of 2.5 or higher. In clinical practice, an abnormally high B/A ratio score may be falsely attributed to cognitive dysfunction. With our digitized pen data, we further explored the causes of the reduced TMT B performance by using new outcome measures, including individual element completion times and interelement variability. These measures indicated selective interference between non-dominant hand use and executive functions. Both non-dominant hand use and performance of the TMT B seem to draw on the same, limited higher-order cognitive resources.