The main goal of this work is to determine whether a computer mouse can be used as a low-cost device for the acquisition of two-dimensional human movement velocity signals in the context of psychophysical studies and biomedical applications. A comprehensive overview of the related literature is presented, and the problem of characterizing mouse movement acquisition is analyzed and discussed. Then, the quality of velocity signals acquired with this kind of device is measured on horizontal oscillatory movements by comparing the mouse data to the signals acquired simultaneously by a video motion tracking system and a digitizing tablet. A synthesis of the information gathered in this work indicates that the computer mouse can be used for the reliable acquisition of biosignals in the context of human movement studies, particularly for many applications dealing with the velocity of the end effector of the upper limb. This paper concludes by discussing the possibilities and limitations of such use.