Does gender affect reactions to violations of expected conversational behavior? This study examined ratings of interactants involved in interruptive exchanges. Audio recordings of two-person interactions that varied in gender composition but were identical in script features were rated by judges on several scales, including the degree to which participants were seen to be argumentative, rude, and assertive. Results showed that interrupter sex did not affect ratings even though interrupters were evaluated differently than those they interrupted. However, gender composition significantly affected two of three derived factors, disrespect and assertiveness, such that when a woman interrupted a man, the pair was rated significantly more disrespectful and assertive than either of the two same-sex pairs. Conversational interruptions that occur among mixed-sex pairs are often interpreted not merely as individual infractions but as an assault on the established power relations.