Introduction: Blood flow restriction (BFR) walking elicits improved fitness, but participants often report higher ratings of perceived exertion (RPE) and pain during BFR walking compared to non-BFR walking. The primary aim was to investigate how multiple BFR walking exposures might affect RPE and pain. Methods: 14 healthy, trained participants completed three BFR walking sessions on separate days. The treadmill speed that elicited 3/10 RPE while BFR was not applied was determined and that same speed was used during all BFR walking sessions. Participants walked for 15 minutes at the predetermined speed while 60% limb occlusion pressure was applied bilaterally to the thighs. RPE (0-10) and pain (0-10) were recorded during each minute of exercise. Two-way repeated measures analysis of variance determined if session (1-3) and/or time (1-15 minutes) affected RPE or pain. Statistical significance was established at p<0.05. Results: RPE was higher during session 1 compared to session 2 (minutes 8-15, 4.4±1.4 versus 3.6±0.8). RPE was higher during session 1 compared to session 3 (minutes 3-5, 3.5±0.7 versus 2.9±0.6; minutes 7-15, 4.3±1.3 versus 3.5±1.1). No significant differences were observed for pain. Conclusion: Participants might tolerate BFR walking better after completing two BFR walking sessions as lowered RPE responses were observed.