Sleep consolidates memory for emotional events. Whether it likewise preserves or, to the contrary, depotentiates emotional responses associated with such events is unclear, as there is evidence to support both cases. Conflicting findings may be partially due to differences in the way emotional reactivity is measured. Thus, the goal of the current project is to investigate the influence of sleep on emotional reactivity using multiple simultaneous measures. Young adults (18–30 years) viewed 45 negative and 45 neutral pictures before a period containing either a nap measured with polysomnography (n=33) or wake (n=25). Following the nap or wake period, they viewed the same pictures intermixed with novel ones and indicated whether they remembered each picture. Emotional response to each picture was measured at both time points with valence and arousal ratings, skin conductance response, heart rate deceleration, and corrugator supercilii electromyography (corrugator EMG). Across the sessions, both valence ratings (t=2.043, p=0.046) and skin conductance response (t=-4.052, p<0.001) were preserved for negative pictures in the nap group relative to the wake group. Arousal ratings, heart rate deceleration, and corrugator EMG response declined non-significantly more in the wake group than the nap group. Participants in the nap group remembered non-significantly more pictures than those in the wake group (M+/-SD: 85.25+/-10.14% vs. 80.83+/-10.56%). Polysomnography data will be analyzed to investigate the relationship between specific features of sleep and change in reactivity measures. These data suggest that sleep consolidates reactivity associated with emotional experiences whether it is measured with subjective rating scales or objective physiological responses. This work was funded by NIH R01 AG040133 (PI: Spencer).