The article deals with the issue of regional lexical units (LU) processed in the Pralex lexical database (LDB), specifically with the processing of regionally marked lexical units in the rising dictionaries while defining regionalisms of different type in the Czech tradition and in the Pralex LDB. It also emphasizes the problems occurring during the processing of this type of LU in the LDB. Furthermore, it examines the shifts in our evaluation of regional LU in comparison with the previous dictionaries (Dictionary of the Standard Czech Language, Dictionary of Standard Czech, Academy Dictionary of Loanwords in Czech).