The aim of the present paper was to study heart rate changes during a video stimulation depicting two actors (male and female) producing dynamic facial expressions of happiness, sadness, and a neutral expression. We measured ballistocardiographic emotion-related heart rate responses with an unobtrusive measurement device called the EMFi chair. Ratings of subjective responses to the video stimuli were also collected. The results showed that the video stimuli evoked significantly different ratings of emotional valence and arousal. Heart rate decelerated in response to all stimuli and the deceleration was the strongest during negative stimulation. Furthermore, stimuli from the male actor evoked significantly larger arousal ratings and heart rate responses than the stimuli from the female actor. The results also showed differential responding between female and male participants. The present results support the hypothesis that heart rate decelerates in response to films depicting dynamic negative facial expressions. The present results also support the idea that the EMFi chair can be used to perceive emotional responses from people while they are interacting with technology.